Journal: Toxicological Sciences

Article Title: Nonclinical safety assessment of SBT777101, a CAR-engineered regulatory T cell therapy for autoimmune diseases

doi: 10.1093/toxsci/kfag125

Figure Lengend Snippet: Selective activation of SBT777101 Jurkat reporter cells by citrullinated proteins. Representative dose–response curves showing fold-increase in luminescence ( y -axis) relative to baseline following incubation with increasing concentrations (log scale, x -axis) of citrullinated (red) or noncitrullinated (black) protein targets. Data points represent the mean ± SD of 3 replicates.

Article Snippet: NFAT-luciferase reporter Jurkat cells (BPS Bioscience) were stably transduced with the SBT777101 CAR.

Techniques: Activation Assay, Incubation

Journal: Molecules

Article Title: Cell Reporter-Based Analysis of Anti-Inflammasome Activities of Flavonoids with Distinct Structures

doi: 10.3390/molecules31183176

Figure Lengend Snippet: Evaluation of the HEK-Blue IL-1R reporter platform for IL-1β detection. ( A ) Experimental protocol used to assess the dynamic range in response to IL-1β. ( B ) Statistical comparison among IL-1β standards ranging from 0.01 to 10,000 pg/mL as measured by the HEK-Blue IL-1R system. ( C ) Nonlinear four-parameter logistic (4PL) regression model generated from IL-1β standards. ( D ) Experimental workflow to obtain IL-1β-containing supernatants from BMMs in the presence or absence of LPS (10 ng/mL) and SEAP quantification using the HEK-Blue IL-1R assay. ( E ) Detection of SEAP activity over the course of a 5–15 min incubation with QUANTI-Blue reagent. N = 3, **** p < 0.0001, ns = not significant.

Article Snippet: HEK-Blue IL-1R reporter cells were obtained from InvivoGen (San Diego, CA, USA).

Techniques: Comparison, Generated, Activity Assay, Incubation

Journal: Molecules

Article Title: Cell Reporter-Based Analysis of Anti-Inflammasome Activities of Flavonoids with Distinct Structures

doi: 10.3390/molecules31183176

Figure Lengend Snippet: Application of the IL-1R HEK-Blue platform to compare the effects of flavonoids. ( A ) Experimental workflow for the detection of IL-1β in supernatants from BMMs treated with flavonoids and/or LPS using IL-1R HEK-Blue cells. ( B ) Comparison of SEAP activity, measured as OD at 640 nm, in Hek-Blue supernatants following exposure to media from BMMs cultured with or without LPS (10 ng/mL, 24 h). ( C ) Effects of flavonoid pretreatment (5–50 µM; NOB, QUER, or RUT) on SEAP induction by supernatants from LPS-treated BMMs. The −LPS and +LPS dashed lines indicate the mean OD values at 10 min from panel ( B ). ( D ) Comparison of OD values in Hek-Blue cell supernatants following exposure to supernatants from BMMs pretreated with flavonoids (10 µM). N = 12 for the −LPS and + LPS treatment groups and n = 4 for the flavonoid pretreatment groups. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: HEK-Blue IL-1R reporter cells were obtained from InvivoGen (San Diego, CA, USA).

Techniques: Comparison, Activity Assay, Cell Culture